Periodically apply a thin layer of centrifuge grease to the rubber O-rings on the rotor lid to maintain a proper vacuum seal. 6. Troubleshooting and Error Codes
Understanding the physical and operational limits of the Eppendorf 5402 ensures safe operation and reproducible experimental results. 14,000 RPM (Rotations Per Minute)
When the Eppendorf 5402 encounters an issue, it utilizes internal sensors to halt operation and protect your samples. Error Messages & Solutions Problem / Error Likely Cause Recommended Action Lid not closed completely, or power supply disconnected.
Unbalanced loads cause severe vibrations that can bend the drive shaft or shatter the rotor housing. If the machine shakes violently upon startup, press Stop immediately. eppendorf 5402 manual
Maximum speed of 14,000 RPM, achieving up to 16,000 x g. Rapid Cooling: Approx. 10 minutes from
5.1 Available Rotors (Fixed-angle, Swing-bucket – if any) 5.2 Maximum Load and Speed per Rotor 5.3 Adapters and Buckets 5.4 Rotor Life and Replacement
The centrifuge will accelerate smoothly to the set speed. The timer will begin counting down once the set speed is achieved. 3. Cycle Completion and Sample Retrieval Periodically apply a thin layer of centrifuge grease
The Eppendorf 5402 is a legacy refrigerated microcentrifuge designed for temperature-sensitive laboratory work. While finding a direct "story" or the full 5402 manual online can be difficult due to its age, it shares many operational features with current Eppendorf models like the 5420 or 5424 Key Specifications & Features Temperature Range: Adjustable from Fast Cool Function: Can cool the rotor chamber to within approximately 10 minutes. Features variable speeds up to 14,000 rpm Typically accommodates up to 18 tubes ( Digital control ranging from 1 to 99 minutes. GMI - Trusted Laboratory Solutions General Operating Instructions
The rotor is still spinning, or the electronic latch has failed.
The manual's operational flowchart is simple but precise: 14,000 RPM (Rotations Per Minute) When the Eppendorf
Adjust the speed dial to the required RPM or RCF.
If samples are spilled, clean the rotor immediately. The F-45-18-11 rotor is susceptible to corrosion from aggressive chemicals.
Releases the motorized lid lock. This button only functions when the rotor has come to a complete, absolute standstill.
Regular upkeep extends the lifespan of the refrigerated system and mechanical components. Routine Cleaning
Proper placement of the centrifuge prevents operational hazards and ensures the longevity of the refrigeration compressor. Location Requirements